Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain
Genet. mol. biol
;
26(2): 213-220, Jun. 2003. tab, graf
Article
in English
| LILACS
| ID: lil-345973
ABSTRACT
The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation
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Index:
LILACS (Americas)
Main subject:
Rec A Recombinases
/
Recombination, Genetic
/
Saccharomyces cerevisiae
/
DNA Repair
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Pernambuco/BR
/
Universidade Federal do Rio Grande do Sul/BR
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