Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay
Mem. Inst. Oswaldo Cruz
;
98(6): 849-851, Sept. 2003. tab, graf
Article
in English
| LILACS
| ID: lil-348358
RESUMO
The present study was conducted to detected IgG antibodies using Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by the enzyme-linked immunosorbent assay (ELISA). Sera from 90 subjects were analyzed (30 with strongyloidiasis, 30 with other parasites and 30 healthy individuals). Results were expressed in antibody titers, which were considered as positive when titer was > 80. Sensibility and specificity of the assay were 100 percent and 96.7 percent, respectively. It can be concluded that the heterologous alkaline extract could be employed in ELISA as a diagnostic aid in human strongyloidiasis, due to its advantages as easiness of obtaining, practicability in preparing, and high indexes of sensitivity and specificity
Full text:
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Index:
LILACS (Americas)
Main subject:
Strongyloides
/
Strongyloidiasis
/
Antibodies, Helminth
/
Antigens, Helminth
Type of study:
Diagnostic study
/
Observational study
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdade de Ciências Farmacêuticas de Ribeiräo Preto/BR
/
Universidade Estadual de Campinas/BR
/
Universidade Federal de Uberlândia/BR
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