Mutación puntual del gen supresor de tumores TP53 en lesiones preneoplásicas y neoplásicas del estómago / Mutations of TP53 suppressor gene in pre-neoplastic and neoplastic lesions of the stomach: cross-sectional study in a high risk region
Rev. méd. Chile
;
131(4): 359-365, abr. 2003. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-348362
ABSTRACT
Background:
In the current model for the development of gastric cancer, regions of multifocal atrophic gastritis give rise to intestinal metaplasia, dysplasia and finally, adenocarcinoma.Aim:
To study the frequency and characteristics of TP53 gene mutations in preneoplastic and neoplastic lesions of the stomach. Material andmethods:
DNA sequencing of the TP53 gene was performed in 46 patients with gastric carcinoma. Normal mucosa, intestinal metaplasia and invasive adenocarcinoma tissues were obtained by scraping 6-Ám histological sections from formalin-fixed and paraffin-embedded tissue.Results:
DNA sequencing of exons 5-9 of the TP53 gene demonstrated a mutation in 31 percent of patients. These findings were seen both in tumoral tissue (13 cases) and in intestinal metaplasia (2 cases). Most mutations were found in exons 5 and 8, and the majority of them were transitions (10 out of 19 mutations).Discussion:
Patients with gastric cancer showed a frequency of TP53 mutations similar to that previously communicated in populations with low gastric cancer risk. Moreover, there was a predominance of transitions, genetic alterations that are identified with carcinogenesis associated with N-nitrosamine compounds. Finally, mutations of TP53 gene were detected in areas of intestinal metaplasia
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Stomach Neoplasms
/
Suppression, Genetic
/
Genes, p53
Type of study:
Etiology study
/
Observational study
/
Prevalence study
/
Prognostic study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2003
Type:
Article
/
Project document
Affiliation country:
Chile
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