Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil
Braz. j. microbiol
;
33(3): 274-278, July-Sept. 2002. ilus
Article
in English
| LILACS
| ID: lil-349783
RESUMO
This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by co-transfection of a deletion fragment containing the 5 and 3 gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Vulvovaginitis
/
In Vitro Techniques
/
Vaccines
/
Glycoproteins
/
Herpesviridae Infections
/
Herpesvirus 1, Bovine
/
Infectious Bovine Rhinotracheitis
Type of study:
Evaluation studies
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2002
Type:
Article
Affiliation country:
Brazil
/
Netherlands
Institution/Affiliation country:
Centro de Pesquisas Veterinárias Desidério Finamor/BR
/
Division of Infectious Diseases and Food Chain Quality/NL
/
Universidade Federal de Santa Maria/BR
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