Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Arq. bras. med. vet. zootec
;
55(3): 301-308, jun. 2003. ilus, tab
Article
in English
| LILACS
| ID: lil-350609
RESUMO
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000æm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000æm cut interval) to 300,830+111,460 (at the 500æm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6æm to 27.8æm.
Full text:
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Index:
LILACS (Americas)
Main subject:
Cebus
/
Ovarian Follicle
Limits:
Animals
Language:
English
Journal:
Arq. bras. med. vet. zootec
Journal subject:
Veterinary Medicine
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Ministério da Saúde/BR
/
Universidade Estadual do Ceará/BR
/
Universidade Federal do Pará/BR
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