Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
Braz. j. infect. dis
;
7(5): 297-300, Oct. 2003. tab
Article
in English
| LILACS
| ID: lil-354278
ABSTRACT
In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34 percent. GABHS was diagnosed in 30 percent of the bacterial cultures, in 25 percent of the samples tested with the molecular nucleic acid hybridization method and in 26 percent of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Streptococcal Infections
/
Streptococcus
/
Pharyngitis
/
Tonsillitis
/
Bacterial Typing Techniques
Type of study:
Diagnostic study
/
Prevalence study
/
Prognostic study
/
Risk factors
Limits:
Child
/
Child, preschool
/
Humans
/
Infant
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2003
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS