Evaluation of four methods for detecting the beta-lactamase activity in Neisseria gonorrhoeae isolated in Cuba
Mem. Inst. Oswaldo Cruz
;
98(8): 1089-1091, Dec. 2003. tab
Article
in English
| LILACS
| ID: lil-355740
RESUMO
Four methods (chromogenic, acidimetric, inhibition, and iodometric) for demonstration of the beta-lactamase production by 70 isolates of Neisseria gonorrhoeae, were evaluated in Cuba. There was 100 percent correlation between all beta-lactamase methods and the standardized penicillin dilution susceptibility test for penicillinase-non-producing N. gonorrhoeae. For penicillinase-producing N. gonorrhoeae strains, there was a perfect correlation between the chromogenic method and penicillin susceptibility testing, but one and two strains failed to give a positive result for beta-lactamase with the inhibition/acidimetric and the iodometric methods, respectively. There was a high concordance between the chromogenic method, considered as gold standard and the rest of penicillinase tests evaluated Kappa Index (KI) = 0.98 for inhibition/acidimetric methods and KI = 0.97 for the iodometric method. The four methods evaluated were accurate, reproducible, easily readable, economical, and ease to use for screening primary isolates of N. gonorrhoeae in Cuba. We recommended the use of the inhibition method, when testing the penicillinase activity in gonococcal isolates in provincial and municipal reference laboratories.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Beta-Lactamases
/
Bacteriological Techniques
/
Neisseria gonorrhoeae
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Caribbean
/
Cuba
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2003
Type:
Article
Affiliation country:
Cuba
Institution/Affiliation country:
Instituto de Medicina Tropical Pedro Kourí/CU
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