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Detection of Cryptosporidium - specific coproantigen in human immunodeficiency virus/acquired immunodeficiency syndrome patients by using a commercially available immunoenzymatic assay
Silva, Claudio Vieira; Ferreira, Marcelo Simão; Gonçalves-Pires, Maria do Rosário de Fátima; Costa-Cruz, Julia Maria.
  • Silva, Claudio Vieira; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Parasitologia. Uberlândia. BR
  • Ferreira, Marcelo Simão; Universidade Federal de Uberlândia. Faculdade de Medicina. Uberlândia. BR
  • Gonçalves-Pires, Maria do Rosário de Fátima; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Parasitologia. Uberlândia. BR
  • Costa-Cruz, Julia Maria; Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Parasitologia. Uberlândia. BR
Mem. Inst. Oswaldo Cruz ; 98(8): 1097-1099, Dec. 2003. graf
Article in English | LILACS | ID: lil-355742
ABSTRACT
The aim of this study was to verify the occurrence of Cryptosporidium infection in 52 human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients (group 1) and 38 clinically healthy individuals (group 2) by using enzyme immunoassay (EIA). All fecal samples collected were submitted to the Baermann, Lutz, and Ritchie methods, the Safranin/Methylene Blue, and Weber's chromotrope modified Trichrome staining techniques, and EIA. In group 1, parasitological staining techniques and EIA were both positive for Cryptosporidium sp. infection in 3/52 (5.8 percent) samples and both negative in 45/52 (86.5 percent) samples, while 4/52 (7.7 percent) samples were positive in EIA and negative in parasitological staining techniques. Concerning group 2, all samples were negative by EIA and microscopy for Cryptosporidium infection. In conclusion, EIA may be an alternative method for detecting Cryptosporidium-specific coproantigen in HIV/AIDS patients.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Reagent Kits, Diagnostic / AIDS-Related Opportunistic Infections / Cryptosporidiosis / Cryptosporidium / Diarrhea Type of study: Diagnostic study / Observational study Limits: Animals / Female / Humans / Male Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2003 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Uberlândia/BR

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Full text: Available Index: LILACS (Americas) Main subject: Reagent Kits, Diagnostic / AIDS-Related Opportunistic Infections / Cryptosporidiosis / Cryptosporidium / Diarrhea Type of study: Diagnostic study / Observational study Limits: Animals / Female / Humans / Male Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2003 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Federal de Uberlândia/BR