Plasminogen interaction with Trypanosoma cruzi
Mem. Inst. Oswaldo Cruz
;
99(1): 63-67, Feb. 2004. ilus, graf
Article
in English
| LILACS
| ID: lil-356445
RESUMO
The ability of Trypanosoma cruzi to interact with plasminogen, the zimogenic form of the blood serin protease plasmin, was examined. Immunohistochemistry studies revealed that both forms, epimastigotes and metacyclic trypomastigotes, were able to fix plasminogen in a lysine dependant manner. This interaction was corroborated by plasminogen activation studies. Both forms of the parasite enhanced the plasminogen activation by tissue-type plasminogen activator.The maximal enhancements obtained were 15-fold and 3.4-fold with epimastigotes and metacyclic trypomastigotes, respectively, as compared to plasminogen activation in absence of cells. Ligand-blotting analysis of proteins extracted with Triton X-114 from a microsomal fraction of epimastigotes revealed at least five soluble proteins and one hydrophobic protein able to bind plasminogen.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plasminogen
/
Trypanosoma cruzi
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
/
Project document
Affiliation country:
Venezuela
Institution/Affiliation country:
Universidad de Los Andes/VE
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