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Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum
Cervelatti, Eliane P; Ferreira-Nozawa, Monica S; Aquino-Ferreira, Roseli; Fachin, Ana Lúcia; Martinez-Rossi, Nilce M.
Affiliation
  • Cervelatti, Eliane P; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto. BR
  • Ferreira-Nozawa, Monica S; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto. BR
  • Aquino-Ferreira, Roseli; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto. BR
  • Fachin, Ana Lúcia; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto. BR
  • Martinez-Rossi, Nilce M; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Genética. Ribeirão Preto. BR
Genet. mol. biol ; Genet. mol. biol;27(1): 99-102, 2004. ilus, tab
Article in En | LILACS | ID: lil-357880
Responsible library: BR26.1
RESUMO
The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number and size of T. rubrum chromosomes, these results open perspectives for the construction of chromosome-specific libraries and for the physical mapping of genes of interest, thus permitting future gene linkage studies in this pathogen. A detailed understanding of the karyotype and genomic organization of T. rubrum should contribute to further genetic, taxonomic and epidemiological studies of this dermatophyte.
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Full text: 1 Index: LILACS Main subject: Trichophyton / Chromosome Mapping / Dermatomycoses Limits: Humans Language: En Journal: Genet. mol. biol / Genet. mol. biol. (Online) / Genetics and molecular biology (Impresso) Journal subject: GENETICA Year: 2004 Type: Article
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Full text: 1 Index: LILACS Main subject: Trichophyton / Chromosome Mapping / Dermatomycoses Limits: Humans Language: En Journal: Genet. mol. biol / Genet. mol. biol. (Online) / Genetics and molecular biology (Impresso) Journal subject: GENETICA Year: 2004 Type: Article