Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA
Mem. Inst. Oswaldo Cruz
;
99(2): 147-152, Mar. 2004. ilus, tab
Article
in English
| LILACS
| ID: lil-360967
ABSTRACT
The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Candida
/
DNA, Fungal
Type of study:
Controlled clinical trial
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Universidade Estadual Paulista/BR
/
Universidade Federal de Minas Gerais/BR
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