Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)
Mem. Inst. Oswaldo Cruz
;
99(2): 153-158, Mar. 2004. ilus, tab
Article
in English
| LILACS
| ID: lil-360968
ABSTRACT
The first and second internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA of Biomphalaria tenagophila complex (B. tenagophila, B. occidentalis, and B. t. guaibensis) were sequenced and compared. The alignment lengths of these regions were about 655 bp and 481 bp, respectively. Phylogenetic relationships among the Biomphalaria species were inferred by Maximum Parsimony and Neighbor-joining methods. The phylogenetic trees produced, in most of the cases, were in accordance with morphological systematics and other molecular data previously obtained by polymerase chain reaction and restriction fragment length polymorphism analysis. The present results provide support for the proposal that B. tenagophila represents a complex comprising B. tenagophila, B. occidentalis and B. t. guaibensis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Phylogeny
/
Biomphalaria
/
DNA, Ribosomal
/
DNA, Helminth
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
/
Project document
Affiliation country:
Argentina
/
Brazil
/
United States
Institution/Affiliation country:
Fiocruz/BR
/
Instituto Ludwig de Pesquisa sobre o Câncer/BR
/
Ludwig Institue for Cancer Research/US
/
Universidad de Buenos Aires/AR
/
Universidade Federal de Minas Gerais/BR
/
University of Georgia/US
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