Actividad antimicrobiana de actinomycetes aislados desde ambientes acuáticos del sur de Chile / Antimicrobial activity of actinomycetes isolated from aquatic environments in Southern Chile
Rev. méd. Chile
;
132(2): 151-159, feb. 2004. mapas, tab, graf
Article
in Spanish
| LILACS
| ID: lil-361490
ABSTRACT
Background:
The easy access and inappropriate use of antimicrobials led to selection and spread of resistant microorganisms strains. It is imperative to search for new and more effective antimicrobials. One strategy is the screening of metabolites produced by microorganisms found in the environment. Actinomycetes are a potential source of new drugs.Aim:
To isolate actinomycetes from sediments of Chilean rivers and lakes and to screen them for antimicrobial activity against reference bacterial strains and pathogenic fungi. Material andMethods:
Actinomycetes were isolated from sediment samples, using casein-starch agar. The antimicrobial activity against 3 bacterial species and 7 fungal species was tested using the disc diffusion method. For the extraction of active metabolites, culture broths of antagonistic actinomycetes were extracted with organic solvents followed by testing the antibiotic activity.Results:
A total of 62 strains of actinomycetes were isolated, mainly Streptomyces sp (83.9 percent). Thirty six strains (58.1 percent) showed antimicrobial activity, mainly against Bacillus subtilis and Candida albicans. Some isolates inhibited a wide spectrum of indicator strains, like LRI 4A strain (Streptomyces sp) that inhibited Bacillus subtilis, Candida albicans and 4 filamentous fungi.Conclusions:
Lakes and rivers of Southern Chile are an important reservoir of antagonistic actinomycetes, a potential source of new drugs.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Actinobacteria
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2004
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Universidad Austral de Chile/CL
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