Pfcrt and pfmdr1 alleles associated with chloroquine resistance in Plasmodium falciparum from Guyana, South America
Mem. Inst. Oswaldo Cruz
;
99(4): 389-392, Jun. 2004. tab
Article
in English
| LILACS
| ID: lil-363856
RESUMO
Using DNA extracted from 112 parasitised blood blots, we screened for the population marker of chloroquine resistance (CQR) pfcrt K76T in Plasmodium falciparum infections from Guyana. Pfmdr1 mutations S1034C, N1042D, and D1246Y also associated with CQR were surveyed as well in 15 isolates for which the in vitro responses to CQ were known. Results indicate that the pfcrt K76T is ubiquitous in this environment, and confirmatory sequencing of codons 72 and 76 revealed two novel allelic sequences SVMIT and RVMNT in addition to the previously identified CVMNT and SVMNT haplotypes. The frequency of the pfcrt K76T despite its presence in both CQR and CQS (chloroquine sensitive) infections measured in vivo and in vitro, suggests that it is a useful population marker in this low-transmission setting of sweeping CQR.
Full text:
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Index:
LILACS (Americas)
Main subject:
Plasmodium falciparum
/
Drug Resistance
/
Chloroquine
/
Antimalarials
Type of study:
Risk factors
Limits:
Humans
Country/Region as subject:
South America
/
English Caribbean
/
Guyana
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
Affiliation country:
Trinidad and Tobago
Institution/Affiliation country:
University of the West Indies/TT
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