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Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil
Rodrigues, Márcia Alves Vasconcelos; Serafini, Alvaro Bisol; Pereira, Marieta de Souza; Silva, Thathiane Dias da; Rabahi, Marcelo Fouad; Alves, Suely Lemes de; Kipnis, André.
  • Rodrigues, Márcia Alves Vasconcelos; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Departamento de Microbiologia. Goiânia. BR
  • Serafini, Alvaro Bisol; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Departamento de Microbiologia. Goiânia. BR
  • Pereira, Marieta de Souza; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Departamento de Microbiologia. Goiânia. BR
  • Silva, Thathiane Dias da; Secretaria Estadual da Agricultura. BR
  • Rabahi, Marcelo Fouad; Secretaria Estadual de Saúde. Hospital de Doenças Tropicais. BR
  • Alves, Suely Lemes de; Laboratório Central. Goiânia. BR
  • Kipnis, André; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Departamento de Microbiologia. Goiânia. BR
Mem. Inst. Oswaldo Cruz ; 99(4): 415-419, Jun. 2004. ilus, tab
Article in English | LILACS | ID: lil-363860
Responsible library: BR1.1
ABSTRACT
This study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6 percent) were negative by the 3 methods, 2 (2.8 percent) were smear positive and negative by culture and in-house PCR, 11 (15.3 percent) were both smear and culture negative, and in-house PCR positive, 7 (9.7 percent) were positive by the 3 methods, 2 (2.8 percent) were positive by smear and culture, and negative by PCR, 2 (2.8 percent) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8 percent and 81.9 percent, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Pulmonary / Polymerase Chain Reaction / Mycobacterium tuberculosis Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2004 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Laboratório Central/BR / Secretaria Estadual da Agricultura/BR / Secretaria Estadual de Saúde/BR / Universidade Federal de Goiás/BR
Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Pulmonary / Polymerase Chain Reaction / Mycobacterium tuberculosis Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2004 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Laboratório Central/BR / Secretaria Estadual da Agricultura/BR / Secretaria Estadual de Saúde/BR / Universidade Federal de Goiás/BR