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Microtubule cytoskeleton distribution using fluorescent taxoid in tetratrichomonas didelphidis
Lecke, Sheila B; Tasca, Tiana; Souto, André A; Borges, Fernanda P; Wiltusching, Renata C. M; Carli, Gerardo A. de.
  • Lecke, Sheila B; Faculdade de Farmácia. Laboratório de Parasitologia Clínica. Porto Alegre. BR
  • Tasca, Tiana; Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Bioquímica. Porto Alegre. BR
  • Souto, André A; Pontifícia Universidade Católica do Rio Grande do Sul. Faculdade de Química. Laboratório de Síntese Orgânica. Porto Alegre. BR
  • Borges, Fernanda P; Faculdade de Farmácia. Laboratório de Parasitologia Clínica. BR
  • Wiltusching, Renata C. M; Faculdade de Farmácia. Laboratório de Parasitologia Clínica. Porto Alegre. BR
  • Carli, Gerardo A. de; Faculdade de Farmácia. Laboratório de Parasitologia Clínica. Porto Alegre. BR
Parasitol. latinoam ; 58(3/4): 101-105, jul. 2003. ilus
Article in English | LILACS | ID: lil-383486
RESUMO
Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. The specimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative of Taxol which binds to the ab-tubulin polimerized of microtubules of cells. Giemsa stain revealed the morphological features of trichomonads such as four anterior flagella, undulating membrane, axostyle and posterior flagellum. An intense fluorescence was observed in living trophozoites of T. didelphidis and Trichomonas vaginalis (used as control), incubated with FLUTAX-2. An analysis of the composition of the cytoskeleton of T. didelphidis will contribute to understanding the cellular morphology of the parasites. Key words Tetratrichomonas didelphidis, microtubule cytoskeleton, fluorescent taxoid.
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Full text: Available Index: LILACS (Americas) Main subject: Trichomonas / Cytoskeleton / In Vitro Techniques / Fluorescent Dyes / Microtubules Limits: Animals Language: English Journal: Parasitol. latinoam Journal subject: Parasitology Year: 2003 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Farmácia/BR / Pontifícia Universidade Católica do Rio Grande do Sul/BR / Universidade Federal do Rio Grande do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Trichomonas / Cytoskeleton / In Vitro Techniques / Fluorescent Dyes / Microtubules Limits: Animals Language: English Journal: Parasitol. latinoam Journal subject: Parasitology Year: 2003 Type: Article Affiliation country: Brazil Institution/Affiliation country: Faculdade de Farmácia/BR / Pontifícia Universidade Católica do Rio Grande do Sul/BR / Universidade Federal do Rio Grande do Sul/BR