Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Biocell
;
27(2): 213-224, Aug. 2003.
Article
in English
| LILACS
| ID: lil-384241
RESUMO
CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione; CG-NQ), a beta-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 microM CG-NQ, the following effects were observed (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen peroxide. Expression of such effects varied according to hepatocyte samples and taken together strongly support an apoptotic action of CG-NQ dependent on reactive oxygen species.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Naphthoquinones
/
Apoptosis
/
Hepatocytes
Limits:
Humans
/
Male
Language:
English
Journal:
Biocell
Journal subject:
Clulas
Year:
2003
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
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