Identification of immunodominant epitopes of Schistosoma mansoni vaccine candidate antigens using human T cells
Mem. Inst. Oswaldo Cruz
;
99(5,supl.1): 63-66, Aug. 2004. tab, graf
Article
in English
| LILACS
| ID: lil-384481
RESUMO
Paramyosin and Sm14 are two of the six antigens selected by the World Health Organization as candidates to compose a subunit vaccine against schistosomiasis. Both antigens are recognized by individuals naturally resistant to Schistosoma mansoni infection and induced protective immunity in the murine model. Three Sm14 epitopes and eleven paramyosin epitopes were selected by their ability to bind to different HLA-DR molecules using the TEPITOPE computer program, and these peptides were synthetically produced. The cellular recognition of Sm14 and paramyosin epitopes by peripheral blood mononuclear cells of individuals living in endemic area for schistosomiasis was tested by T cell proliferation assay. Among all Sm14 and paramyosin epitopes studied, Sm14-3 was preferentially recognized by individuals naturally resistant to S. mansoni infection while Para-5 was preferentially recognized by individuals resistant to reinfection. These two peptides represent promising antigens to be used in an experimental vaccine against schistosomiasis, since their preferential recognition by resistant individuals suggest their involvement in the induction of protective immunity.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Tropomyosin
/
Schistosomiasis mansoni
/
Vaccines
/
Antigens, Helminth
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Universidade Federal da Bahia/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade de São Paulo/BR
Similar
MEDLINE
...
LILACS
LIS