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Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
Trujillo Toledo, Luis Enrique; Gómez Riera, Raúl; Banguela Castillo, Alexander; Soto Romero, Melvis; Arrieta Sosa, Juan Gabriel; Hernández García, Lázaro.
Affiliation
  • Trujillo Toledo, Luis Enrique; Centro de Ingeniería Genética y Biotecnológica. Laboratorio Interacciobes Planta-Microorganismos. División Plantas. Habana. CU
  • Gómez Riera, Raúl; Centro de Ingeniería Genética y Biotecnológica. División Química Física. Habana. CU
  • Banguela Castillo, Alexander; Centro de Ingeniería Genética y Biotecnológica. Laboratorio Interacciones Planta Microorganismos. División Plantas. Habana. CU
  • Soto Romero, Melvis; Centro de Ingeniería Genética y Biotecnológica. Laboratorio Interacciones Planta Microorganismos. División Plantas. Habana. CU
  • Arrieta Sosa, Juan Gabriel; Centro de Ingeniería Genética y Biotecnológica. Laboratorio Interacciones Planta Microorganismos. División Plantas. Habana. CU
  • Hernández García, Lázaro; Centro de Ingeniería Genética y Biotecnológica. Laboratorio Interacciones Planta Microorganismos. División Plantas. Habana. CU
Electron. j. biotechnol ; 7(2): 115-123, Aug. 2004. ilus, tab, graf
Article in En | LILACS | ID: lil-387552
Responsible library: CL1.1
ABSTRACT
The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures.
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Full text: 1 Index: LILACS Main subject: Oligosaccharides / Pichia / Gluconacetobacter / Fructose / Hexosyltransferases Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2004 Type: Article / Project document
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Full text: 1 Index: LILACS Main subject: Oligosaccharides / Pichia / Gluconacetobacter / Fructose / Hexosyltransferases Language: En Journal: Electron. j. biotechnol Journal subject: BIOTECNOLOGIA Year: 2004 Type: Article / Project document