Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
Mem. Inst. Oswaldo Cruz
;
99(6): 617-620, Oct. 2004. ilus
Article
in English
| LILACS
| ID: lil-387911
RESUMO
Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanocidal Agents
/
Trypanosoma cruzi
/
RNA, Messenger
/
RNA Splicing
/
RNA, Protozoan
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2004
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual Paulista/BR
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