Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela
Mem. Inst. Oswaldo Cruz
;
100(1): 43-46, Feb. 2005. ilus, tab
Article
in English
| LILACS
| ID: lil-398114
ABSTRACT
We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified L. ovallesi (82.75 percent) and L. gomezi (17.42 percent). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of New Word Leishmaniaand Viannia subgenera. The results showed a total of 8 pools (15.68 percent) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Psychodidae
/
Polymerase Chain Reaction
/
DNA, Protozoan
/
Insect Vectors
/
Leishmania
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
Country/Region as subject:
South America
/
Venezuela
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
/
Project document
Affiliation country:
Venezuela
Institution/Affiliation country:
Universidad de Oriente/VE
/
Universidad de los Andes/VE
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