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A shortcut in phage screening technique
Andrade, Alexandre de; Teixeira, Paula Rezende; Siviero, Fábio; Santelli, Roberto Vicente.
  • Andrade, Alexandre de; Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo. BR
  • Teixeira, Paula Rezende; Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo. BR
  • Siviero, Fábio; Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo. BR
  • Santelli, Roberto Vicente; Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo. BR
Genet. mol. biol ; 28(1): 150-151, Jan.-Mar. 2005. ilus, tab
Article in English | LILACS | ID: lil-399631
ABSTRACT
A simple modification of the traditional Benton & Davis technique for phage screening is presented that avoids the tedious sample dilutions of putative spots/phages towards the second screening. With the use of a sole agar plate and nylon filter, the modification distinguishes a true positive recombinant from a false positive, with high probability of success.
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Full text: Available Index: LILACS (Americas) Main subject: Bacteriophages / DNA, Recombinant / Genetic Testing Type of study: Diagnostic study / Screening study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacteriophages / DNA, Recombinant / Genetic Testing Type of study: Diagnostic study / Screening study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade de São Paulo/BR