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Identification of clonally rearranged T-cell receptor beta chain genes in HTLV-I carriers as a potential instrument for early detection of neoplasia
Sales, M. M; Bezerra, C. N. A; Hiraki, Y; Melo, N. B; Rebouças, N. A.
  • Sales, M. M; Hospital das Clínicas. Departamento de Patologia. Divisão de Laboratório Central. Serviço de Hematologia. São Paulo. BR
  • Bezerra, C. N. A; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica. São Paulo. BR
  • Hiraki, Y; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica. São Paulo. BR
  • Melo, N. B; Hemocentro de São Paulo. Fundação Pró-Sangue. São Paulo. BR
  • Rebouças, N. A; Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Fisiologia e Biofísica. São Paulo. BR
Braz. j. med. biol. res ; 38(5): 695-704, May 2005. ilus, tab
Article in English | LILACS | ID: lil-400962
ABSTRACT
We analyzed the genetic recombination pattern of the T-cell receptor beta-chain gene (TCR-beta) in order to identify clonal expansion of T-lymphocytes in 17 human T-lymphotropic virus type I (HTLV-I)-positive healthy carriers, 7 of them with abnormal features in the peripheral blood lymphocytes. Monoclonal or oligoclonal expansion of T-cells was detected in 5 of 7 HTLV-I-positive patients with abnormal lymphocytes and unconfirmed diagnosis by using PCR amplification of segments of TCR-beta gene, in a set of reactions that target 102 different variable (V) segments, covering all members of the 24 V families available in the gene bank, including the more recently identified segments of the Vbeta-5 and Vbeta-8 family and the two diversity beta segments. Southern blots, the gold standard method to detect T-lymphocyte clonality, were negative for all of these 7 patients, what highlights the low sensitivity of this method that requires a large amount of very high quality DNA. To evaluate the performance of PCR in the detection of clonality we also analyzed 18 leukemia patients, all of whom tested positive. Clonal expansion was not detected in any of the negative controls or healthy carriers without abnormal lymphocytes. In conclusion, PCR amplification of segments of rearranged TCR-beta is reliable and highly suitable for the detection of small populations of clonal T-cells in asymptomatic HTLV-I carriers who present abnormal peripheral blood lymphocytes providing an additional instrument for following up these patients with potentially higher risk of leukemia.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Human T-lymphotropic virus 1 / Leukemia-Lymphoma, Adult T-Cell / Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Type of study: Diagnostic study / Prognostic study / Screening study Limits: Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2005 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Hemocentro de São Paulo/BR / Hospital das Clínicas/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Human T-lymphotropic virus 1 / Leukemia-Lymphoma, Adult T-Cell / Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Type of study: Diagnostic study / Prognostic study / Screening study Limits: Adult / Female / Humans / Male Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2005 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Hemocentro de São Paulo/BR / Hospital das Clínicas/BR / Universidade de São Paulo/BR