Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing
Braz. j. infect. dis
;
8(5): 372-377, Oct. 2004. tab
Article
in English
| LILACS
| ID: lil-401707
RESUMO
Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67 percent) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54 percent) isolates were confirmed as ESBL producers, and 18/107 (17 percent) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83 percent) of strains that did not show CAIE. ESBL detection remains a controversial issue and clinical laboratories are in need of a simple and effective way to recognize strains with this kind of resistance.
Full text:
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Index:
LILACS (Americas)
Main subject:
Beta-Lactamases
/
Cefoxitin
/
Escherichia coli
/
Klebsiella pneumoniae
/
Anti-Bacterial Agents
Type of study:
Diagnostic study
/
Practice guideline
/
Screening study
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2004
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal Faculty of Medical Sciences Foundation of Porto Alegre/BR
/
Irmandade Santa Casa de Misericórdia of Porto Alegre/BR
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