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Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis
Beck, S. T; Leite, O. M; Arruda, R. S; Ferreira, A. W.
  • Beck, S. T; Federal University of Santa Maria. Santa Maria. BR
  • Leite, O. M; São Paulo University. Faculty of Medicine. Clinical Hospital. São Paulo. BR
  • Arruda, R. S; Clinical Hospital. São Paulo. BR
  • Ferreira, A. W; Biolab-Merieux S/A. Rio de Janeiro. BR
Braz. j. infect. dis ; 9(1)Feb. 2005. graf
Article in English | LILACS | ID: lil-404306
RESUMO
Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85 percent sensitivity and 91 percent specificity. ELISA sensitivity improved from 85 percent to 92 percent when the Western blot results were used. Western blot specificity was 100 percent when antibody reactivity with different antigenic bands was analyzed and associated. The association of TbF6/DPEP antigens used in ELISA with specific patterns of reactivity determined by Western blot can help make an identification when classic methods for the diagnosis of pulmonary tuberculosis are not sufficient.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Pulmonary / Antibodies, Bacterial / Mycobacterium tuberculosis / Antigens, Bacterial Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Adolescent / Adult / Aged / Humans Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: A+BR / Clinical Hospital/BR / Federal University of Santa Maria/BR / São Paulo University/BR

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Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis, Pulmonary / Antibodies, Bacterial / Mycobacterium tuberculosis / Antigens, Bacterial Type of study: Diagnostic study / Observational study / Prognostic study / Risk factors Limits: Adolescent / Adult / Aged / Humans Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: A+BR / Clinical Hospital/BR / Federal University of Santa Maria/BR / São Paulo University/BR