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Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
Wilhelm, Vivian; Soza, Cristián; Martínez, Rodrigo; Rosemblatt, Mario; Burzio, Luis O; Valenzuela, Pablo D. T.
  • Wilhelm, Vivian; Fundación Ciencia para la Vida. CL
  • Soza, Cristián; Fundación Ciencia para la Vida. CL
  • Martínez, Rodrigo; Fundación Ciencia para la Vida. CL
  • Rosemblatt, Mario; Fundación Ciencia para la Vida. CL
  • Burzio, Luis O; Fundación Ciencia para la Vida. CL
  • Valenzuela, Pablo D. T; Fundación Ciencia para la Vida. CL
Biol. Res ; 38(1): 69-82, 2005. ilus, graf
Article in English | LILACS | ID: lil-404829
RESUMO
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80 percent homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Salmon / DNA, Bacterial / Piscirickettsiaceae Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2005 Type: Article Affiliation country: Chile Institution/Affiliation country: Fundación Ciencia para la Vida/CL

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Full text: Available Index: LILACS (Americas) Main subject: Salmon / DNA, Bacterial / Piscirickettsiaceae Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2005 Type: Article Affiliation country: Chile Institution/Affiliation country: Fundación Ciencia para la Vida/CL