The biological effects of high-pressure gas on the yeast transcriptome
Braz. j. med. biol. res
;
38(8): 1267-1272, Aug. 2005. ilus, tab
Article
in English
| LILACS
| ID: lil-405530
RESUMO
The aim of the present study was to examine the feasibility of DNA microarray technology in an attempt to construct an evaluation system for determining gas toxicity using high-pressure conditions, as it is well known that pressure increases the concentration of a gas. As a first step, we used yeast (Saccharomyces cerevisiae) as the indicator organism and analyzed the mRNA expression profiles after exposure of yeast cells to nitrogen gas. Nitrogen gas was selected as a negative control since this gas has low toxicity. Yeast DNA microarray analysis revealed induction of genes whose products were localized to the membranes, and of genes that are involved in or contribute to energy production. Furthermore, we found that nitrogen gas significantly affected the transport system in the cells. Interestingly, nitrogen gas also resulted in induction of cold-shock responsive genes. These results suggest the possibility of applying yeast DNA microarray to gas bioassays up to 40 MPa. We therefore think that "bioassays" are ideal for use in environmental control and protection studies.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Saccharomyces cerevisiae
/
RNA, Fungal
/
Gene Expression Regulation, Fungal
/
Hydrostatic Pressure
/
Nitrogen
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
2005
Type:
Article
/
Congress and conference
Affiliation country:
Japan
Institution/Affiliation country:
National Institute of Advanced Industrial Science and Technology/JP
/
The University of Tokushima/JP
Similar
MEDLINE
...
LILACS
LIS