Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil
Mem. Inst. Oswaldo Cruz
;
100(4): 371-376, July 2005. ilus, tab
Article
in English
| LILACS
| ID: lil-405991
RESUMO
The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Human T-lymphotropic virus 1
/
HTLV-I Infections
/
HTLV-II Infections
/
HIV Infections
/
HIV-1
Type of study:
Prevalence study
/
Risk factors
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Laboratório Central do Estado do Amapá/BR
/
Unidade de Referência de Doenças Infecciosas e Parasitárias Especiais/BR
/
Universidade Federal do Pará/BR
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