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Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil
Laurentino, R. V; Lopes, I. G. L; Azevedo, V. N; Machado, L. F. A; Moreira, M. R. C; Lobato, L; Ishak, M. O. G; Ishak, R; Vallinoto, A. C. R.
  • Laurentino, R. V; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Lopes, I. G. L; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Azevedo, V. N; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Machado, L. F. A; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Moreira, M. R. C; Laboratório Central do Estado do Amapá. Macapá. BR
  • Lobato, L; Unidade de Referência de Doenças Infecciosas e Parasitárias Especiais. Belém. BR
  • Ishak, M. O. G; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Ishak, R; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
  • Vallinoto, A. C. R; Universidade Federal do Pará. Centro de Ciências Biológicas. Departamento de Patologia. Laboratório de Virologia. Belém. BR
Mem. Inst. Oswaldo Cruz ; 100(4): 371-376, July 2005. ilus, tab
Article in English | LILACS | ID: lil-405991
RESUMO
The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Human T-lymphotropic virus 1 / HTLV-I Infections / HTLV-II Infections / HIV Infections / HIV-1 Type of study: Prevalence study / Risk factors Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Central do Estado do Amapá/BR / Unidade de Referência de Doenças Infecciosas e Parasitárias Especiais/BR / Universidade Federal do Pará/BR

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Full text: Available Index: LILACS (Americas) Main subject: Human T-lymphotropic virus 1 / HTLV-I Infections / HTLV-II Infections / HIV Infections / HIV-1 Type of study: Prevalence study / Risk factors Limits: Female / Humans / Male Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Laboratório Central do Estado do Amapá/BR / Unidade de Referência de Doenças Infecciosas e Parasitárias Especiais/BR / Universidade Federal do Pará/BR