Identification of Anopheles (Nyssorhynchus) albitarsis complex species (Diptera: Culicidae) using rDNA internal transcribed spacer 2-based polymerase chain reaction primes
Mem. Inst. Oswaldo Cruz
;
100(5): 495-500, Aug. 2005.
Article
in English
| LILACS
| ID: lil-409967
ABSTRACT
Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified polymorphic DNA (RAPD) markers, but various other methodologies also point to multiple species under the name An. albitarsis. We describe here a technique for identification of these species employing polymerase chain reaction (PCR) primers based on ribosomal DNA internal transcribed spacer 2 (rDNA ITS2) sequence. Since this method is based on known sequence it is simpler than the sometimes problematical RAPD-PCR. Primers were tested on samples previously identified using RAPD markers with complete correlation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Ribosomal Spacer
/
Insect Vectors
/
Anopheles
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Argentina
/
Brazil
/
Venezuela
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
Affiliation country:
United States
Institution/Affiliation country:
Walter Reed Army Institute of Research/US
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