Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
Mem. Inst. Oswaldo Cruz
;
100(3): 269-272, May 2005. ilus
Article
in English
| LILACS
| ID: lil-411022
ABSTRACT
Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4 percent), followed by genotype 3 (21.4 percent), and genotype 2 (7.2 percent). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4 percent), mild (57.2 percent), and moderate (21.4 percent). Viral RNA was detected in liver cells from nine patients (64.3 percent). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
RNA, Viral
/
In Situ Hybridization
/
Hepacivirus
/
Hepatitis C, Chronic
/
Liver
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
/
Universidade Federal do Rio de Janeiro/BR
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