Leishmania (Viannia) braziliensis growth in vitro culture relies more on folic acid availability than Leihsmania (Leishmania) amazonensis
Mem. Inst. Oswaldo Cruz
;
100(3): 309-310, May 2005.
Article
in English
| LILACS
| ID: lil-411030
ABSTRACT
We compared the in vitro growth of promastigotes from two Leishmania species in TC-100 and Schneider media. Leishmania (Leishmania) amazonensis replication rates were similar in both tissue culture media and reached maximum rates by 48 h. In contrast Leishmania (Viannia) braziliensis growth was significantly greater in TC-100 but maximum rates were achieved by 96 h. Folic acid appears to be the limiting factor and supplementation of Schneider media with this nutrient improved L. (V.) braziliensis replication rates and decreased the time of maximum replication to 48 h.
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Index:
LILACS (Americas)
Main subject:
Culture Media
/
Folic Acid
/
Leishmania
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
/
Project document
Affiliation country:
Colombia
Institution/Affiliation country:
Centro Internacional de Física/CO
/
Universidad Nacional de Colombia/CO
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