Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny
Genet. mol. biol
;
28(2): 294-298, 2005. ilus, tab
Article
in English
| LILACS
| ID: lil-416301
ABSTRACT
We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the alpha = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial artificial chromosome (BAC) clones hybridizing to the sucrose synthase EST were identified.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Expressed Sequence Tags
/
Saccharum
Type of study:
Risk factors
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2005
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Centro de Tecnologia Copersucar/BR
/
Texas A&M University/US
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