Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells
Genet. mol. res. (Online)
;
4(1): 55-66, Mar. 2005.
Article
in English
| LILACS
| ID: lil-417409
ABSTRACT
An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE). There were no significant differences (P > 0.5) in either fusion (116/149 = 78% vs 216/301 = 72%), cleavage (78/116 = 67% vs 141/216 = 65%) and blastocyst (35/116 = 30% vs 52/216 = 24%) rates or in pregnancy rate at 30 to 35 days after embryo transfer (2/17 vs 3/17) between NT using FCE and TFCE, respectively. Transfection and long-term in vitro culture of transfected cells did not affect developmental capacity of NT embryos up to 40 days of gestation
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cattle
/
Animals, Genetically Modified
/
Cell Nucleus
/
Embryo Transfer
/
Fibroblasts
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2005
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Genetic Resources and Biotechnology/BR
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