Molecular cytogenetic assignment of genes to bovine chromosome 5
Genet. mol. res. (Online)
;
2(3): 260-270, Sept. 2003.
Article
in English
| LILACS
| ID: lil-417604
ABSTRACT
Seven genes were assigned by molecular cytogenetic methods to bovine chromosome 5. To accomplish this, specific primers were either publicly available or were designed from highly conserved regions of the publicly available mammalian gene sequences. The identity of the amplified segments was verified by sequencing and alignment with the published sequences. The optimized primers that amplified the desired bovine genes were used for screening a bovine bacterial artificial chromosome library. The positive clones were localized to a specific band of bovine chromosome 5 by fluorescence in situ hybridization. The genes HOXC4, SP1 and IGFBP6 were localized to band q21, COL2A1 was localized to bands q21-q23, IGF1 was localized to band q26, MB to band q31 and the gene CYP2D6 was localized to band q35. The cytogenetic assignment of SP1, IGFBP6, COL2A1, IGF1, MB and CYP2D6 is first reported here and the assignment of HOXC4 refines the previous assignment of this gene. The identification and localization of these genes further support the development of the human to bovine comparative map through characterizing the homologous segments conserved in the evolution of these species. This information will be useful for the future localization of genes that affect economically important traits in bovines
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cattle
/
Chromosome Mapping
/
Quantitative Trait, Heritable
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2003
Type:
Article
Affiliation country:
United States
Institution/Affiliation country:
Texas A and M University/US
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