Molecular characterization of ABC transporter-encoding genes in Aspergillus nidulans
Genet. mol. res. (Online)
;
1(4): 337-349, Dec. 2002.
Article
in English
| LILACS
| ID: lil-417632
ABSTRACT
As a preliminary step towards characterizing genes encoding ATP-binding cassette (ABC) transporters that confer pleiotropic drug resistance in Aspergillus, we used a PCR-based approach to isolate four DNA fragments corresponding to different ABC type transporter genes. DNA sequencing and Southern blot analysis confirmed that they were distinct genes, which were designated abcA-D. One of these genes, abcD, was cloned and characterized. It was found to have a predicted 1,452-amino acid translation product with a calculated molecular mass of 147,467 kDa. The abcD gene specifies a single transcript of approximately 5.0 kb; there was a two- to six-fold enhancement of mRNA levels following exposure to miconazole, camptothecin, methotrexate, and ethidium bromide
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Index:
LILACS (Americas)
Main subject:
Aspergillus nidulans
/
ATP-Binding Cassette Transporters
/
Drug Resistance, Multiple, Fungal
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2002
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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