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Neutralization of the neuromuscular activity of bothropstoxin-I, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (Guaçatonga
Oshima-Franco, Y.; Alves, C. M. V.; Andréo Filho, N.; Gerenutti, M.; Cintra, A. C. O.; Leite, G. B.; Rodrigues-Simioni, L.; Silva, M. G..
  • Oshima-Franco, Y.; University of Sorocaba. School of Pharmacy. Sorocaba. BR
  • Alves, C. M. V.; University of Sorocaba. School of Pharmacy. Sorocaba. BR
  • Andréo Filho, N.; University of Sorocaba. School of Pharmacy. Sorocaba. BR
  • Gerenutti, M.; University of Sorocaba. School of Pharmacy. Sorocaba. BR
  • Cintra, A. C. O.; University of São Paulo. School of Pharmaceutical Sciences. Ribeirão Preto. BR
  • Leite, G. B.; State University of Campinas. Department of Pharmacology. Campinas. BR
  • Rodrigues-Simioni, L.; State University of Campinas. Department of Pharmacology. Campinas. BR
  • Silva, M. G.; University of Sorocaba. School of Pharmacy. Sorocaba. BR
J. venom. anim. toxins incl. trop. dis ; 11(4): 465-478, out.-dez. 2005. graf
Article in English | LILACS | ID: lil-417720
RESUMO
Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 mg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37°C) of BthTX-I (20 mg/ml, n=4) with a concentration of the hydroalcoholic extract (4 mg/ml) that had no neuromuscular activity, such as the control (n=5), prevented the neuromuscular blockade caused by the toxin. This protection may be mediated by compounds such as flavonoids and phenols identified by thin-layer chromatography and colorimetric assays
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Plants, Medicinal / Snake Bites / Snake Venoms / Plant Extracts Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR / University of Sorocaba/BR / University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plants, Medicinal / Snake Bites / Snake Venoms / Plant Extracts Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: State University of Campinas/BR / University of Sorocaba/BR / University of São Paulo/BR