Padronizacão da técnica de imunoistoquímica para o diagnóstico etiológico de rotina da diarréia bovina a vírus / Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine
Arq. bras. med. vet. zootec
;
57(5): 710-714, out. 2005. ilus
Article
in Portuguese
| LILACS
| ID: lil-418854
RESUMO
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cattle
/
Immunohistochemistry
/
Diarrhea Viruses, Bovine Viral
/
Diagnostic Tests, Routine
Type of study:
Diagnostic study
/
Etiology study
Language:
Portuguese
Journal:
Arq. bras. med. vet. zootec
Journal subject:
Veterinary Medicine
Year:
2005
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal de Minas Gerais/BR
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