Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil
Mem. Inst. Oswaldo Cruz
;
100(7): 739-741, Nov. 2005. ilus
Article
in English
| LILACS
| ID: lil-419699
RESUMO
A natural case of co-infection by Leishmania and Trypanosoma is reported in a dog (Canis familiaris) in south- western state of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms were observed after Giemsa staining of cytological preparations of the dog's bone marrow aspirate. No parasite was detected using medium culture inoculation of the sample. DNA obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (PCR) with a set of rDNA-based primers S4/S12. The nucleotide sequence of the PCR product was identical to that of Trypanosoma (Trypanozoon) evansi. The S4/S12 PCR was then used as template in a nested-PCR using a specific Leishmania set S17/S18 as primers, to explain the amastigote forms. The nucleotide sequence of the new PCR product was identical to that of Leishmania (Leishmania) chagasi. This case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides L. (L.) chagasi, the natural transmission of T. (T.) evansi occurs in the area under study.
Full text:
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Index:
LILACS (Americas)
Main subject:
Trypanosomiasis
/
Dog Diseases
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
Faculdade de Saúde Pública/BR
/
Prefeitura do Município de São Paulo/BR
/
Universidade de São Paulo/BR
/
Universidade para o Desenvolvimento do Estado e da Região do Pantanal/BR
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