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Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
Araújo, Flábio R; Melo, Valeska S. P; Ramos, Carlos A. N; Madruga, Claudio R; Soares, Cleber O; Kessler, Raul H; Almeida, Nalvo F; Araújo, Graziela S; Alves, Leucio C; Torres Júnior, Roberto A. A; Fragoso, Stênio P; Arauco, Paulo R. C; Bacanelli, Gisele; Oliveira, Maristeli B; Santos, Lenita R.
  • Araújo, Flábio R; Embrapa Gado de Corte. Campo Grande. BR
  • Melo, Valeska S. P; Universidade Federal Rurual de Pernambuco. Departamento de Medicina Veterinária. Recife. BR
  • Ramos, Carlos A. N; Embrapa Gado de Corte. Campo Grande. BR
  • Madruga, Claudio R; Embrapa Gado de Corte. Campo Grande. BR
  • Soares, Cleber O; Embrapa Gado de Corte. Campo Grande. BR
  • Kessler, Raul H; Embrapa Gado de Corte. Campo Grande. BR
  • Almeida, Nalvo F; Universidade Federal do Mato Grosso do Sul. Departamento de Computacão e Estatística. Campo Grande. BR
  • Araújo, Graziela S; Universidade Federal do Mato Grosso do Sul. Departamento de Computacão e Estatística. Campo Grande. BR
  • Alves, Leucio C; Universidade Federal Rurual de Pernambuco. Departamento de Medicina Veterinária. Recife. BR
  • Torres Júnior, Roberto A. A; Embrapa Gado de Corte. Campo Grande. BR
  • Fragoso, Stênio P; Instituto de Biologia Molecular do Paraná. Curitiba. BR
  • Arauco, Paulo R. C; Instituto de Biologia Molecular do Paraná. Curitiba. BR
  • Bacanelli, Gisele; Embrapa Gado de Corte. Campo Grande. BR
  • Oliveira, Maristeli B; Embrapa Gado de Corte. Campo Grande. BR
  • Santos, Lenita R; Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador. BR
Mem. Inst. Oswaldo Cruz ; 100(7): 765-769, Nov. 2005. ilus, tab
Article in English | LILACS | ID: lil-419704
ABSTRACT
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99 percent for both tests) and specificities (100 percent for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.
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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Outer Membrane Proteins / Enzyme-Linked Immunosorbent Assay / Cattle Diseases / Anaplasma marginale / Anaplasmosis / Antibodies, Bacterial / Antigens, Bacterial Type of study: Diagnostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Embrapa Gado de Corte/BR / Instituto de Biologia Molecular do Paraná/BR / Universidade Federal Rurual de Pernambuco/BR / Universidade Federal da Bahia/BR / Universidade Federal do Mato Grosso do Sul/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacterial Outer Membrane Proteins / Enzyme-Linked Immunosorbent Assay / Cattle Diseases / Anaplasma marginale / Anaplasmosis / Antibodies, Bacterial / Antigens, Bacterial Type of study: Diagnostic study Limits: Animals Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2005 Type: Article Affiliation country: Brazil Institution/Affiliation country: Embrapa Gado de Corte/BR / Instituto de Biologia Molecular do Paraná/BR / Universidade Federal Rurual de Pernambuco/BR / Universidade Federal da Bahia/BR / Universidade Federal do Mato Grosso do Sul/BR