Clinical and pathological importance of vacA allele heterogeneity and cagA status in peptic ulcer disease in patients from north Brazil
Mem. Inst. Oswaldo Cruz
;
100(8): 875-881, Dec. 2005. tab
Article
in English
| LILACS
| ID: lil-419954
ABSTRACT
We have examined the prevalence of gene cagA and vacA alleles in 129 patients, 69 with gastritis and 60 with peptic ulcer diseases from North Brazil and their relation with histopathological data. vacA and cagA genotype were determined by polymerase chain reaction. Hematoxylin-eosin staining was used for histological diagnosis. 96.6 percent of the patients were colonized by Helicobacter pylori strains harboring single vacA genotype (nont-mixed infection). Among them, 11.8 percent had subtype s1a, 67.8 percent had subtype s1b, and 17 percent subtype s2. In regard to the middle region analysis, m1 alleles were found in 75.4 percent and m2 in 21.2 percent of patients. The cagA gene was detected in 78 percent patients infected with H. pylori and was associated with the s1-m1 vacA genotype. The H. pylori strains, vacA s1b m1/cagA-positive, were associated with increased risk of peptic ulcer disease and higher amounts of lymphocytic and neutrophilic infiltrates and the presence of intestinal metaplasia. These findings show that cagA and vacA genotyping may have clinical relevance in Brazil.
Full text:
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Index:
LILACS (Americas)
Main subject:
Peptic Ulcer
/
Bacterial Proteins
/
Helicobacter pylori
/
Helicobacter Infections
/
Gastritis
/
Antigens, Bacterial
Type of study:
Risk factors
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2005
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Hospital Ofir Loiola/BR
/
Hospital Universitário João de Barros Barreto/BR
/
Universidade Federal do Pará/BR
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