Recombinant tegumental protein Shistosoma japonicum very lowdensity lipoprotein binding protein as a vaccine candidate against Schistosoma japonicum
Mem. Inst. Oswaldo Cruz
;
101(1): 9-13, Feb. 2006. graf
Article
in English
| LILACS
| ID: lil-423560
RESUMO
A polyhistidine-tagged recombinant tegumental protein Schistosoma japonicum very lowdensity lipoprotein binding protein (SVLBP) from adult Schistosoma japonicum was expressed in Escherichia coli. The affinity purified rSVLBP was used to vaccinate mice. The worm numbers and egg deposition recovered from the livers and veins of the immunized mice were 33.5 percent and 47.6 percent less than that from control mice, respectively (p<0.05). There was also a marked increase in the antibody response in vaccinated mice the titer of IgG1 and IgG2a, IgG2b in the vaccinated group was significantly higher than that in the controls (>16,400 in total IgG). In a comparison of the reactivity of sera from healthy individuals and patients with rSVLBP, recognition patterns against this parasite tegumental antigen varied among different groups of the individuals. Notably, the average titres of anti-rSVLBP antibody in sera from faecal egg-negative individuals was significantly higher than that in sera from the faecal egg-positives, which may be reflect SVLBP-specific protection. These results suggested that the parasite tegumental protein SVLBP was a promising candidate for further investigation as a vaccine antigen for use against Asian schistosomiasis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma japonicum
/
Recombinant Proteins
/
Antibodies, Helminth
/
Vaccines, Synthetic
/
Histidine
/
Lipoproteins, VLDL
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Australia
/
China
/
United States
Institution/Affiliation country:
Tulane University Health Sciences Center/US
/
University of Queensland/AU
/
Zhejiang Academy of Medical Sciences/CN
Similar
MEDLINE
...
LILACS
LIS