Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence
Mem. Inst. Oswaldo Cruz
;
101(1): 39-45, Feb. 2006. ilus, tab, mapas
Article
in English
| LILACS
| ID: lil-430838
RESUMO
Triatoma venosa presents a restricted geographical distribution in America and is considered as a secondary vector of Chagas disease in Colombia and Ecuador. A total of 120 adult insects were collected in domestic and peridomestic habitats in an endemic area of the department of Boyacá, Colombia, in order to determine their genetic structure through morphometric and molecular techniques. The head and wings of each specimen were used for the analyses of size, shape, and sexual dimorphism. A significant sexual dimorphism was found, although no differences in size among the studied groups were detected. Differences were found in the analyzed structures except for male heads. DNA was extracted from the legs in order to carry out the internal transcriber space-2 (ITS-2) amplification and the randon amplified polymorphic DNA (RAPD) analyses. Length polymorphisms were not detected in the ITS-2. Fst and Nm values were estimated (0.047 and 3.4, respectively). The high genetic flow found among the insects captured in the domicile and peridomiciliary environment does not permit a genetic differentiation, thus establishing the peridomicile as an important place for epidemiological surveillance.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Triatoma
/
Sex Characteristics
/
DNA, Ribosomal Spacer
/
Insect Vectors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
/
Project document
Affiliation country:
Colombia
/
Spain
Institution/Affiliation country:
Universidad de Valencia/ES
/
Universidad de los Andes/CO
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