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Genetic characterization of St. Louis encephalitis virus isolated from human in São Paulo, Brazil
Santos, Cecília Luiza Simões dos; Sallum, Maria Anice Mureb; Franco, Heitor Moreira; Oshiro, Fabíola Maiumi; Rocco, Iray Maria.
  • Santos, Cecília Luiza Simões dos; Instituto Adolfo Lutz. São Paulo. BR
  • Sallum, Maria Anice Mureb; Universidade de São Paulo. Faculdade de Saúde Pública. Departamento de Epidemiologia. São Paulo. BR
  • Franco, Heitor Moreira; Instituto Adolfo Lutz. São Paulo. BR
  • Oshiro, Fabíola Maiumi; Instituto Adolfo Lutz. São Paulo. BR
  • Rocco, Iray Maria; Instituto Adolfo Lutz. São Paulo. BR
Mem. Inst. Oswaldo Cruz ; 101(1): 57-63, Feb. 2006. tab
Article in English | LILACS | ID: lil-430841
Responsible library: BR1.1
RESUMO
The molecular characterization of SPH253157, a new strain of St. Louis encephalitis virus (SLEV), isolated in 2004 from the first case of human infection recognized in the state of São Paulo, Brazil, is reported. The patient, presenting a febrile illness without neurological involvement, was hospitalized as a probable case of dengue fever. Genomic RNA was isolated from the supernatant of C6/36 cells infected with acute phase-serum specimen of the patient and the envelope gene was amplified by reverse-transcription-polymerase chain reaction. The complete nucleotide sequence of the envelope gene of this isolate was directly sequenced from the amplified products and compared with other Brazilian and American SLEV strains. Phylogenetic analyses were carried out under maximum likelihood criterion with outgroups both included and excluded. Outgroups comprised four flavivirus of the Japanese encephalitis group. Phylogeny also included Bayesian analysis. The results indicated that the new SLEV isolate belongs to lineage III, being closely related to an Argentinean strain recovered from Culex sp. in 1979. It is concluded that there are at least 3 lineages of SLEV in Brazil.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: RNA, Viral / Viral Envelope Proteins / Encephalitis Virus, St. Louis Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2006 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: RNA, Viral / Viral Envelope Proteins / Encephalitis Virus, St. Louis Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2006 Type: Article / Project document Affiliation country: Brazil Institution/Affiliation country: Instituto Adolfo Lutz/BR / Universidade de São Paulo/BR