Evaluation of polymerase chain reaction for the detection of Paracoccidioides brasiliensis DNA on serum samples from patients with paracoccidioidomycosis
Mem. Inst. Oswaldo Cruz
;
101(2): 219-221, Mar. 2006.
Article
in English
| LILACS
| ID: lil-430900
RESUMO
The aim of this study was to demonstrate the DNA of Paracoccidioides brasiliensis in human serum samples of patients with paracoccidioidomycosis using the polymerase chain reaction (PCR). The diagnosis of paracoccidioidomycosis (PCM) was defined by microscopic observation of the fungus on direct exam or histopathology, culture, and serological positivity. DNA from serum of 33 patients with PCM was extracted and submitted to nested-PCR using primers from the gp 43 gene. Only one sample was positive on nested-PCR. We conclude that the prevalence of fungemia in patients with different clinical forms of PCM is low, limiting the use of serum DNA detection as an alternative diagnostic tool.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Paracoccidioides
/
Paracoccidioidomycosis
/
DNA, Fungal
/
Fungal Proteins
/
Glycoproteins
/
Polymerase Chain Reaction
/
Fungemia
/
Antigens, Fungal
Type of study:
Diagnostic study
/
Risk factors
Limits:
Adult
/
Child
/
Female
/
Humans
/
Male
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto de Medicina Tropical de São Paulo/BR
/
Instituto de Medicina Tropical de São PauloI/BR
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