In vitro susceptibility to antifungal agents of environmental Cryptococcus spp. isolated in the city of Ribeirão Preto, São Paulo, Brazil
Mem. Inst. Oswaldo Cruz
;
101(3): 239-243, May 2006. tab
Article
in English
| LILACS
| ID: lil-431719
RESUMO
Infections by Cryptococcus strains other than C. neoformans have been detected in immunocompromised patients. Of these strains, three are considered human pathogens: C. albidus, C. laurenttii, and C. uniguttulatus. This study deals with the in vitro susceptibility of Cryptococcus to drugs such as amphotericin B, itraconazole, fluconazole, and 5-fluorocytosine. Environmental Cryptococcus isolates (50) distributed as follows: C. neoformans var. neoformans (16), C. albidus (17), C. laurentii (14), and C. uniguttulatus (3) were evaluated by the micro and macrodilution techniques, according to EUCAST and NCCLS recommendations, respectively. Considering both methodologies the respective minimal inhibitory concentrations (MIC) were 0.125 and 2 æg/ml for amphotericin B, 0.06 and 8 æg/ml for itraconazole, and 0.5 and more than 64 æg/ml for fluconazole and 5-fluorocytosine. Agreement percentages for the two methodologies were 100 percent for amphotericin B and fluconazole for all the strains tested. For itraconazole, the agreement percentage was 81.3 percent in the C. neoformans strain and 100 percent for all the others. All species had a agreement percentage of 94.1 to 100 percent when susceptibility to 5-fluorocytosine was tested. It is concluded that environmental isolates of C. neoformans var. neoformans, C. albidus, C. laurentii, and C. uniguttulatus may show high MICs against certain drugs, suggesting in vitro primary resistance to the antifungals tested.
Full text:
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Index:
LILACS (Americas)
Main subject:
Cryptococcus
/
Environmental Microbiology
/
Antifungal Agents
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade de São Paulo/BR
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