A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)
Mem. Inst. Oswaldo Cruz
;
101(3): 339-340, May 2006. ilus
Article
in English
| LILACS
| ID: lil-431737
ABSTRACT
Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus and Triatoma lecticularia. The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses.
Full text:
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Index:
LILACS (Americas)
Main subject:
Rhodnius
/
Testis
/
Triatoma
/
Chromosomes
/
Cytogenetic Analysis
/
Insect Vectors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Universidad de Antioquia/CO
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