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Citric acid production from glucose by yeast Candida oleophila ATCC 20177 under batch, continuous and repeated batch cultivation
Anastassiadis, Savas; Rehm, Hans-Jürgen.
  • Anastassiadis, Savas; Research in Biotechnology. Thessaloniki. GR
  • Rehm, Hans-Jürgen; University of Münster. Institute of Molecular Microbiology and Biotechnology. Münster. DE
Electron. j. biotechnol ; 9(1)Jan. 2006.
Article in English | LILACS | ID: lil-432456
ABSTRACT
The effect of air saturation on citric acid production in batch, repeated batch and chemostat cultures has been studied. It was shown that, under continuous fermentation (chemostat mode), the highest concentration of citric acid equal of 98 g/l was produced at 20% of air saturation. In contrary to continuous fermentation, displaying an optimum at 20%, 80% air saturation yielded higher values in repeated batch fermentation process. 167 g/l citric acid were produced continuously with the fill and drain technique at 4.85 days, at 80% air saturation, compared with 157.6 g/l achieved within 5.4 days at 20%. Under repeated batch fermentation, the formation rate of the generic product (Rj) as well as the specific citric acid productivity (mp) reached a maximum of 1.283 g/(l x hr) at 4.01 days and of 0.0375 g/(g x hr) at 4.58 days, respectively. The glucose consumption rate (Rx) reached a maximum value of 3.33 g/(l x hr) entering stationary phase after 2.56 days at a glucose concentration of 131.2 g/l.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Oxygen / Yeasts / Citric Acid / Glucose Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2006 Type: Article Affiliation country: Germany / Greece Institution/Affiliation country: Research in Biotechnology/GR / University of Münster/DE

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Full text: Available Index: LILACS (Americas) Main subject: Oxygen / Yeasts / Citric Acid / Glucose Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2006 Type: Article Affiliation country: Germany / Greece Institution/Affiliation country: Research in Biotechnology/GR / University of Münster/DE