A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
Electron. j. biotechnol
;
9(1)Jan. 2006.
Article
in English
| LILACS
| ID: lil-432462
ABSTRACT
For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Polymorphism, Restriction Fragment Length
/
DNA, Plant
/
Random Amplified Polymorphic DNA Technique
/
Terminalia
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2006
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Hamdard University/IN
/
India Habitat Center/IN
/
University of Delhi South Campus/IN
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