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A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis
Sarwat, Maryam; Singh Negi, Madan; Lakshmikumaran, Malathi; Kumar Tyagi, Akhilesh; Das, Sandip; Shankar Srivastava, Prem.
  • Sarwat, Maryam; Hamdard University. Department of Biotechnology. New Delhi. IN
  • Singh Negi, Madan; India Habitat Center. New Delhi. IN
  • Lakshmikumaran, Malathi; India Habitat Center. New Delhi. IN
  • Kumar Tyagi, Akhilesh; University of Delhi South Campus. Department of Plant Molecular Biology. New Delhi. IN
  • Das, Sandip; Hamdard University. Department of Biotechnology. New Delhi. IN
  • Shankar Srivastava, Prem; Hamdard University. Department of Biotechnology. New Delhi. IN
Electron. j. biotechnol ; 9(1)Jan. 2006.
Article in English | LILACS | ID: lil-432462
ABSTRACT
For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).
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Full text: Available Index: LILACS (Americas) Main subject: Genetic Variation / Polymorphism, Restriction Fragment Length / DNA, Plant / Random Amplified Polymorphic DNA Technique / Terminalia Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2006 Type: Article Affiliation country: India Institution/Affiliation country: Hamdard University/IN / India Habitat Center/IN / University of Delhi South Campus/IN

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Full text: Available Index: LILACS (Americas) Main subject: Genetic Variation / Polymorphism, Restriction Fragment Length / DNA, Plant / Random Amplified Polymorphic DNA Technique / Terminalia Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2006 Type: Article Affiliation country: India Institution/Affiliation country: Hamdard University/IN / India Habitat Center/IN / University of Delhi South Campus/IN