Quantitative determination of ciprofloxacin and norfloxacin in pharmaceutical preparations by high performance liquid chromatography

RESUMO

The objetive of the research was to develop and validate an analytical method for quantitative determination of ciprofloxacin (CIP) and norfloxacin (NOR) in pharmaceutical preparations. A simple and rapid chromatographic method was developed and validated for quantitative determination of two fluoroquinolone antibiotics in tablets and injection preparations. The quinolones were analyzed by using a LiChrospher® 100 RP-18 column (5 µm, 125 x 4 mm) and a mobile phase consisted of wateracetronitriletriethylamine (80200.3 v/v/v). The pH of final mixture was adjusted to 3.3 with phosphoric acid. The flow rate was 1.0 mL/min and UV detection was made at 279nm. The analyses were performed at room temperature (24 ± 2°C). CIP and NOR were eluted within 5 min. The calibration curves were linear (r > 0.9999) over a concentration range from 4.0 to 24.0 µg/mL. The relative standard deviation (RSD) was < 1.0 percent and the mean recovery was 101.85 percent...