Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
J. venom. anim. toxins incl. trop. dis
;
12(2): 202-214, 2006. ilus
Article
in English
| LILACS
| ID: lil-434718
ABSTRACT
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agents DNA was not detected in any sample of the other experimental groups. New studies must be carried out to verify the technique sensitivity in researches on this agents genetic material using sera samples of acute-phase toxoplasmosis patients, especially in cases of immunosuppression
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Rats
/
Serologic Tests
/
Toxoplasmosis
/
Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
São Paulo State University/BR
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